# Malaria thin blood film

Microscopy analysis of Giemsa-stained thick and thin blood films from peripheral blood is intended to detect, identify, and quantify malaria parasites.

**Thin films** are similar to usual blood films and allow species identification because the parasite's appearance is best preserved in this preparation.

![ Diagram of slide reading](https://357738419-files.gitbook.io/~/files/v0/b/gitbook-legacy-files/o/assets%2F-MJNQBgYLwFTXBIbt1TJ%2F-MJhWQrKrhS3Z8Axh_-6%2F-MJhWRMOvL_EVu0zCxfU%2Fimage.png?alt=media\&token=3b546e49-6d67-477e-8442-12424f0f47f5)

![Malaria parasites in the thin film](https://357738419-files.gitbook.io/~/files/v0/b/gitbook-legacy-files/o/assets%2F-MJNQBgYLwFTXBIbt1TJ%2F-MJhWkQNeKIJA0UAMzFk%2F-MJhXep_tJU_6zth1Bk6%2Fimage.png?alt=media\&token=50c67170-f45a-453c-b9f8-8e9377dc82a7)

{% hint style="warning" %}
The procedure requires 100× oil-immersion objective lens
{% endhint %}

## Malaria thin, using Celly

#### Preparation 

Put the sample, turn on the light, use 100x objective lens magnification, and set the lens focus. Move the field of view to the single layer of cells.

#### Test execution

Tap the test configuration button: set "**Malaria Thin: parasites counting**" test type, choose a patient. 

Tap the app settings button. Set the cell number to analyze (10000 by default). Start the test.

Move your slide, controlling the focus. The App will count red blood cells and probably infected cells. After reaching the specified number of cells, the application will complete the test.

{% hint style="info" %}
Check at least 5000 red blood cells to calculate the parasitemia level
{% endhint %}

#### Result review and confirmation

* Log in on the [**website**](https://app.celly.ai). Go to the [**Tests**](https://app.celly.ai/app/analysis/) page. Push the **View** button to open your report.
* Go to the **Cells** tab to check the pre-classification. Drag the cells to the appropriate type to correct errors. 
* The app automatically calculates **Parasitemia level** (Infected cells per 1000 RBC) on the slide

How to prepare the slides - <https://youtu.be/vo-JmxTEtk4>&#x20;

Video Tutorial - [Malaria Parasitemia Counting (Thin Film)](https://youtu.be/abS-qmueUoE)