Malaria thin blood film

Microscopy analysis of Giemsa-stained thick and thin blood films from peripheral blood is intended to detect, identify, and quantify malaria parasites.

Thin films are similar to usual blood films and allow species identification because the parasite's appearance is best preserved in this preparation.

Diagram of slide reading

Malaria parasites in the thin film

The procedure requires 100× oil-immersion objective lens

Malaria thin, using Celly

Preparation

Put the sample, turn on the light, use 100x objective lens magnification, and set the lens focus. Move the field of view to the single layer of cells.

Test execution

Tap the test configuration button: set "Malaria Thin: parasites counting" test type, choose a patient.

Tap the app settings button. Set the cell number to analyze (10000 by default). Start the test.

Move your slide, controlling the focus. The App will count red blood cells and probably infected cells. After reaching the specified number of cells, the application will complete the test.

Check at least 5000 red blood cells to calculate the parasitemia level

Result review and confirmation

• Log in on the website. Go to the Tests page. Push the View button to open your report.

• Go to the Cells tab to check the pre-classification. Drag the cells to the appropriate type to correct errors.

• The app automatically calculates Parasitemia level (Infected cells per 1000 RBC) on the slide

How to prepare the slides - https://youtu.be/vo-JmxTEtk4

Video Tutorial - Malaria Parasitemia Counting (Thin Film)